tagging of fertility restorer loci using recessive class analysis in wild abortive cytoplasmic male sterility system in rice

Authors

سعید یاراحمدی

محمد مهدی سوهانی

ابوبکر جوهرعلی

بابک ربیعی

علی اکبر عبادی

abstract

tagging of restorer genes for wild abortive (wa) of cytoplasmic male sterility was carried out by studying 10000 individuals from an f2 population of a cross between ir58025a × ir42686r. this study was primarily carried out to reduce the distance of the already known markers for rf gene. this f2 population was identified to be unique as four rf genes could be tagged to ssr markers on chromosomes 1, 7, 10, & 12. the rf3 gene on chromosome 1 was flanked on either side with rm443 and rm315 at a genetic distance of 3.3 and 20.2 cm, respectively. rf6 located on chromosome 10, was flanked with tightly linked ssr markers on either side at a genetic distance of 2.2 and 4.3 cm. here a new molecular marker rm519 is reported to be linked to rf7 on chromosome 12 at a genetic distance of 7.8 cm. moreover, rm6344 was confirmed to be linked to rf4 on chromosome 7 at a genetic distance of 12.2 cm. tagging of closely linked flanking ssr markers to four rf genes will be extremely useful for breeders to have this source screened. in addition, pyramiding of each rf genes with other known sources will be an excellent provision for developing super restorers

Upgrade to premium to download articles

Already have an account?login

similar resources

INHERITANCE OF FERTILITY RESTORATION INVOLVING WILD ABORTIVE CYTOPLASMIC MALE STERILITY SYSTEM IN RICE (Oryza sativa L.)

Inheritance of fertility restoration was studied in crosses involving ten elite restorer lines of rice viz. BR6839-41-5-1R, BR7013-62-1-1R, BR7011-37-1-2R, BR10R, BR11R, BR12R, BR13R, BR14R, BR15R and BR16R and one male sterile line Jin23A with WA sources of cytoplasmic male sterility. The segregation pattern for pollen fertility of F2 and BC1 populations of crosses involving Jin23A indicated t...

full text

The Possible Role of Atp6 Gene in Cytoplasmic Male Sterility in WA (Wild Abortive) Type of Rice (Oryza sativa L.)

The possibility of occurrence of some rearrangements inside and or around coxII and atp6 genes and their relationship with male sterility in rice lines having wild abortive (WA) cytoplasm was studied. Two sterile (IR58025A and IR62829A) and two maintainer lines (IR58025B and IR62829B) were used. Radioactive Southern blotting was employed to carry out the experiments. The hybridization of coxII...

full text

The rice restorer Rf4 for wild-abortive cytoplasmic male sterility encodes a mitochondrial-localized PPR protein that functions in reduction of WA352 transcripts.

full text

the possible role of atp6 gene in cytoplasmic male sterility in wa (wild abortive) type of rice (oryza sativa l.)

the possibility of occurrence of some rearrangements inside and or around coxii and atp6 genes and their relationship with male sterility in rice lines having wild abortive (wa) cytoplasm was studied. two sterile (ir58025a and ir62829a) and two maintainer lines (ir58025b and ir62829b) were used. radioactive southern blotting was employed to carry out the experiments. the hybridization of coxii...

full text

Suppressed expression of Retrograde-Regulated Male Sterility restores pollen fertility in cytoplasmic male sterile rice plants.

Conflict/reconciliation between mitochondria and nuclei in plants is manifested by the fate of pollen (viable or nonviable) in the cytoplasmic male sterility (CMS)/fertility restoration (Rf) system. Through positional cloning, we identified a nuclear candidate gene, RETROGRADE-REGULATED MALE STERILITY (RMS) for Rf17, a fertility restorer gene for Chinese wild rice (CW)-type CMS in rice (Oryza s...

full text

Transfer of Cytoplasmic Male Sterility into Rice

Transfer of Cytoplasmic Male Sterility into Rice Diversifying the existing cytoplasmic male sterility (CMS) sources of rice is indispensable for a sustainable production system of hybrid seed. Ahmadikhah et al. report that maintainer line Yosen B was converted to a CMS line (Yosen A) in three generations by markerassisted backcrossing. The recurrent parent genome content of the final CMS line w...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}

Journal title:

علوم گیاهان زراعی ایران

جلد ۴۱، شماره ۱، صفحات ۰-۰

Keywords

[ ' t a g g i n g o f r e s t o r e r g e n e s f o r w i l d a b o r t i v e ( w a ) o f c y t o p l a s m i c m a l e s t e r i l i t y w a s c a r r i e d o u t b y s t u d y i n g 1 0 0 0 0 i n d i v i d u a l s f r o m a n f 2 p o p u l a t i o n o f a c r o s s b e t w e e n i r 5 8 0 2 5 a × i r 4 2 6 8 6 r . t h i s s t u d y w a s p r i m a r i l y c a r r i e d o u t t o r e d u c e t h e d i s t a n c e o f t h e a l r e a d y k n o w n m a r k e r s f o r r f g e n e . t h i s f 2 p o p u l a t i o n w a s i d e n t i f i e d t o b e u n i q u e a s f o u r r f g e n e s c o u l d b e t a g g e d t o s s r m a r k e r s o n c h r o m o s o m e s 1 ' , 7 , 1 0 , ' & 1 2 . t h e r f 3 g e n e o n c h r o m o s o m e 1 w a s f l a n k e d o n e i t h e r s i d e w i t h r m 4 4 3 a n d r m 3 1 5 a t a g e n e t i c d i s t a n c e o f 3 . 3 a n d 2 0 . 2 c m ' , ' r e s p e c t i v e l y . r f 6 l o c a t e d o n c h r o m o s o m e 1 0 ' , ' w a s f l a n k e d w i t h t i g h t l y l i n k e d s s r m a r k e r s o n e i t h e r s i d e a t a g e n e t i c d i s t a n c e o f 2 . 2 a n d 4 . 3 c m . h e r e a n e w m o l e c u l a r m a r k e r r m 5 1 9 i s r e p o r t e d t o b e l i n k e d t o r f 7 o n c h r o m o s o m e 1 2 a t a g e n e t i c d i s t a n c e o f 7 . 8 c m . m o r e o v e r ' , ' r m 6 3 4 4 w a s c o n f i r m e d t o b e l i n k e d t o r f 4 o n c h r o m o s o m e 7 a t a g e n e t i c d i s t a n c e o f 1 2 . 2 c m . t a g g i n g o f c l o s e l y l i n k e d f l a n k i n g s s r m a r k e r s t o f o u r r f g e n e s w i l l b e e x t r e m e l y u s e f u l f o r b r e e d e r s t o h a v e t h i s s o u r c e s c r e e n e d . i n a d d i t i o n ' , ' p y r a m i d i n g o f e a c h r f g e n e s w i t h o t h e r k n o w n s o u r c e s w i l l b e a n e x c e l l e n t p r o v i s i o n f o r d e v e l o p i n g s u p e r r e s t o r e r s ' ]

Hosted on Doprax cloud platform doprax.com